FITC Plus Anti-Human HLA-DR (L243)

• 

  FC experiment of human PBMCs using FITC-65218

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD14, and 5 ul FITC Plus Anti-Human HLA-DR (L243) (FITC-65218, Clone: L243) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-65218

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human HLA-DR (L243) (FITC-65218, Clone: L243)(red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

HLA-DR Monoclonal Antibody for FC

Cat No. FITC-65218

Clone No.L243

Publications(2)

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

FC

Synonyms

HLA-DRA, HLA DRA, HLA DRA1, HLA DRB, HLA-DRA1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

FITC-65218 targets HLA-DR in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human lymphoblastoid B-cell line RPMI 8866

• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• ENSEMBL Gene ID: ENSG00000204287
• RRID: AB_2924139
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01903
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	FC	Cells; Exosomes from Human Omental Adipose-Derived Mesenchymal Stem Cells Secreted into Ascites Promote Peritoneal Metastasis of Epithelial Ovarian Cancer; Authors - Qingxi Qu
human	FC	Front Immunol; Profiling hypoxia signaling reveals a lncRNA signature contributing to immunosuppression in high-grade glioma; Authors - Xinqiao Li