Tested Applications
| Positive WB detected in | COLO 320 cells, human small intestine tissue, Caco-2 cells, HT-29 cells, human colon tissue |
| Positive IHC detected in | human colon tissue, human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
66798-1-Ig targets HPGD in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1499 Product name: Recombinant human HPGD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-266 aa of BC018986 Sequence: MHVNGKVALVTGAAQGIGRAFAEALLLKGAKVALVDWNLEAGVQCKAALDEQFEPQKTLFIQCDVADQQQLRDTFRKVVDHFGRLDILVNNAGVNNEKNWEKTLQINLVSVISGTYLGLDYMSKQNGGEGGIIINMSSLAGLMPVAQQPVYCASKHGIVGFTRSAALAANLMNSGVRLNAICPGFVNTAILESIEKEENMGQYIEYKDHIKDMIKYYGILDPPLIANGLITLIEDDALNGAIMKITTSKGIHFQDYDTTPFQAKTQ Predict reactive species |
| Full Name | hydroxyprostaglandin dehydrogenase 15-(NAD) |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC018986 |
| Gene Symbol | HPGD |
| Gene ID (NCBI) | 3248 |
| RRID | AB_2861358 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15428 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HPGD antibody 66798-1-Ig | Download protocol |
| IF protocol for HPGD antibody 66798-1-Ig | Download protocol |
| IHC protocol for HPGD antibody 66798-1-Ig | Download protocol |
| WB protocol for HPGD antibody 66798-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS One Molecular mechanism of inhibitory effects of melatonin on prostate cancer cell proliferation, migration and invasion. | ||
Nat Chem Biol ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism
| ||
Reprod Sci Progressively Diminished Prostaglandin E2 Signaling in Concordance with Increasing Fibrosis in Ectopic Endometrium |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Huajun (Verified Customer) (09-11-2023) | Three bands show up for my human macrophage lysate when doing WB. ~27kD band will be the specific signal.
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