GRP94 Monoclonal antibody

GRP94 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, CoIP,ELISA

Conjugate

Unconjugated

CloneNo.

1H10B7

Cat no : 60012-1-Ig

Synonyms

ECGP, GP96, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1



Tested Applications

Positive WB detected inHuman heart, human heart tissue, human liver tissue, human lung tissue, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells, MCF-7 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60012-1-Ig targets GRP94 in WB, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen GRP94 fusion protein Ag1439
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight 96 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC009195
Gene symbol HSP90B1
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

Protocols

Product Specific Protocols
WB protocol for GRP94 antibody 60012-1-IgDownload protocol
IHC protocol for GRP94 antibody 60012-1-IgDownload protocol
IF protocol for GRP94 antibody 60012-1-IgDownload protocol
FC protocol for GRP94 antibody 60012-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Front Physiol

Evidence for Sexual Dimorphism in the Response to TLR3 Activation in the Developing Neonatal Mouse Brain: A Pilot Study.

Authors - Raul Chavez-Valdez
mouseWB

Cell Rep

Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity.

Authors - Fangfang Sun
humanWB

J Adv Res

Development of a novel thymidylate synthase (TS) inhibitor capable of up-regulating P53 expression and inhibiting angiogenesis in NSCLC.

Authors - Xin-Yang Li
ratCoIP

Neurochem Res

Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1.

Authors - Jiajia Chen