HRP-conjugated Human IgA Monoclonal antibody

• 

  WB analysis using HRP-60099

  50 ug, 25 ug, 12.5 ug human saliva were subjected to SDS PAGE followed by western blot with HRP-60099 (Human IgA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using HRP-60099

  human placenta tissue were subjected to SDS PAGE followed by western blot with HRP-60099 (Human IgA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using HRP-60099

  human plasma were subjected to SDS PAGE followed by western blot with HRP-60099 (Human IgA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Human IgA Monoclonal Antibody for WB

Cat No. HRP-60099

Clone No.3A5A5

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Synonyms

FLJ14473, FLJ35065, FLJ35500, FLJ36402, FLJ39698, FLJ40001, FLJ41548, FLJ41552, FLJ41789, FLJ43248, FLJ43594, FLJ44293, FLJ46028, FLJ46621, FLJ46724, FLJ46811, FLJ46824, FLJ90170, Human IgA, IgA1, IGHA1

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Tested Applications

• Positive WB detected in: human saliva, human placenta tissue, human plasma

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

HRP-60099 targets Human IgA in WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human IgA fusion protein Ag7417
• Full Name: immunoglobulin heavy constant alpha 1
• Calculated Molecular Weight: 496 aa, 53 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC016369
• Gene Symbol: Human IgA Heavy Chain
• Gene ID (NCBI): 3493
• RRID: AB_2883830
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	ACS Nano; Treating and Protecting against Recurrent Vulvovaginal Candidiasis Using the Vaginal Epithelial Cell Membrane-Based Photoimmunotherapeutic Nanoplatform; Authors - Ledan Wang