Anti-Rat IFN-gamma Rabbit Recombinant Antibody

IFN-gamma Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Rat

Applications

FC

Conjugate

Unconjugated

CloneNo.

6K20

Cat no : 98001-1-RR

Synonyms

IFN γ, Ifng, IFNG2, interferon gamma

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Validation Data Gallery

• 

  FC experiment of wistar rat splenocytes using 98001-1-RR

  1X10^6 unstimulated or ConA, PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Wistar rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IFN-gamma (98001-1-RR, Clone: 6K20) and PE-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody. Cells were co-stained with 0.25 ug APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity experiments of 98001-1-RR

  Captured anti Rat IFN-gamma IgG(98001-1-RR) on protein A Biosensor, can bind Human NSD3 with an affinity constant of 2.11 nM as determined in a BLI assay by Gator Prime.

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Tested Applications

• Positive FC detected in: ConA, PMA and ionomycin stimulated Wistar rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98001-1-RR targets IFN-gamma in FC applications and shows reactivity with Rat samples.

• Tested Reactivity: Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interferon gamma
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_138880
• Gene Symbol: Ifng
• Gene ID (NCBI): 25712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Interferon-gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)