FITC Plus Anti-Mouse IL-17F Rabbit Recombinant Antibody

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  FC experiment of C57BL/6 Th17-polarized splenocytes using FITC-98019

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th17-polarized splenocytes were intracellularly stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug FITC Plus Anti-Mouse IL-17F Rabbit RecAb (FITC-98019, Clone: 230026G6). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IL-17F Recombinant Antibody for FC (Intra)

Cat No. FITC-98019

Clone No.230026G6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Il17f, 230026G6, C87042, IL 17F, IL-17

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Tested Applications

• Positive FC (Intra) detected in: PMA, Ionomycin and Brefeldin A treated C57BL/6 Th17-polarized splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98019 targets IL-17F in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-17F fusion protein Eg0671
• Full Name: interleukin 17F
• Calculated Molecular Weight: 17KD
• GenBank Accession Number: NM_145856.2
• Gene Symbol: IL-17F
• Gene ID (NCBI): 257630
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7TNI7-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.