CoraLite®594 Anti-Mouse IL-2 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CL594-98055

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated mouse splenocytes were intracellularly stained with CoraLite® Plus 488 Anti-Mouse CD4 and 0.25 ug CoraLite®594 Anti-Mouse IL-2 Rabbit RecAb (CL594-98055, Clone: 240358A2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of mouse splenocytes using CL594-98055

  1x10^6 PMA, Ionomycin and Brefeldin A treated mouse splenocytes were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse IL-2 Rabbit RecAb (CL594-98055, Clone: 240358A2) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse IL-2 Rabbit RecAb (CL594-98055, Clone: 240358A2) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IL-2 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. CL594-98055

Clone No.240358A2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Il2, 240358A2, Il 2, interleukin 2, Interleukin-2

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Tested Applications

• Positive FC (Intra) detected in: PMA, Ionomycin and Brefeldin A treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98055 targets IL-2 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-2 fusion protein Eg0762
• Full Name: interleukin 2
• Calculated Molecular Weight: 19 kDa
• GenBank Accession Number: NM_008366
• Gene Symbol: IL-2
• Gene ID (NCBI): 16183
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04351
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).