Tested Applications
Positive FC (Intra) detected in | PMA, Ionomycin and Brefeldin A treated mouse splenocytes |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
FITC-98055 targets IL-2 in FC (Intra) applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | IL-2 fusion protein Eg0762 Predict reactive species |
Full Name | interleukin 2 |
Calculated Molecular Weight | 19 kDa |
GenBank Accession Number | NM_008366 |
Gene Symbol | IL-2 |
Gene ID (NCBI) | 16183 |
Conjugate | FITC Plus Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P04351 |
Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).
Protocols
Product Specific Protocols | |
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FC protocol for FITC Plus IL-2 antibody FITC-98055 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |