Biotin-conjugated IgG Lambda Light Chain Polyclonal antibody

IgG Lambda Light Chain Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC

Conjugate

Biotin

Cat no : Biotin-20758

Synonyms

IGLC, IGLC1

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Validation Data Gallery

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  IHC staining of human tonsillitis using Biotin-20758

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-20758 (IgG Lambda Light Chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of Biotin-20758 is IgG Lambda Light Chain Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IgG Lambda Light Chain fusion protein Ag14693
• Full Name: immunoglobulin lambda constant 1 (Mcg marker)
• Calculated Molecular Weight: 233 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC007782
• Gene Symbol: IGLC1
• Gene ID (NCBI): 3537
• RRID: AB_2918941
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the lambda light chain of human IgG.