IgG light chain (lambda) Antibody

IgG light chain (lambda) Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20758-1-AP

Synonyms

IGLC, IGLC1



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 20758-1-AP immunogen is Fusion Protein IgG light chain (lambda) protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IgG light chain (lambda) fusion protein Ag14693
Full Name immunoglobulin lambda constant 1 (Mcg marker)
Calculated molecular weight 233aa,25 kDa
Observed molecular weight 25 kDa
GenBank accession number BC007782
Gene symbol IGLC1
Gene ID (NCBI) 3537
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IgG light chain (lambda) antibody 20758-1-APDownload protocol
IHC protocol for IgG light chain (lambda) antibody 20758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols