JMJD6 Polyclonal antibody, PBS Only

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• JMJD6 Polyclonal antibody, PBS Only (16476-1-PBS)

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  WB analysis of mouse liver using 16476-1-AP (same clone as 16476-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16476-1-AP (JMJD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 16476-1-AP (same clone as 16476-1-PBS)

  IP result of anti-JMJD6 (IP:16476-1-AP, 4ug; Detection:16476-1-AP 1:500) with K-562 cells lysate 1200ug. This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 16476-1-AP (same clone as 16476-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug JMJD6 Polyclonal antibody (16476-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

JMJD6 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Cat No. 16476-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

Lysyl-hydroxylase JMJD6, KIAA0585, Jumonji domain-containing protein 6, JmjC domain-containing protein 6, EC:1.14.11.-

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Product Information

16476-1-PBS targets JMJD6 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9590

  Product name: Recombinant human JMJD6 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-403 aa of BC066654

  Sequence: MNHKSKKRIREAKRSARPELKDSLDWTRHNYYESFSLSPAAVADNVERADALQLSVEEFVERYERPYKPVVLLNAQEGWSAQEKWTLERLKRKYRNQKFKCGEDNDGYSVKMKMKYYIEYMESTRDDSPLYIFDSSYGEHPKRRKLLEDYKVPKFFTDDLFQYAGEKRRPPYRWFVMGPPRSGTGIHIDPLGTSAWNALVQGHKRWCLFPTSTPRELIKVTRDEGGNQQDEAITWFNVIYPRTQLPTWPPEFKPLEILQKPGETVFVPGGWWHVVLNLDTTIAITQNFASSTNFPVVWHKTVRGRPKLSRKWYRILKQEHPELAVLADSVDLQESTGIASDSSSDSSSSSSSSSSDSDSECESGSEGDGTVHRRKKRRTCSMVGNGDTTSQDDCVSKERSSSR

• Full Name: jumonji domain containing 6
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC066654
• Gene Symbol: JMJD6
• Gene ID (NCBI): 23210
• RRID: AB_2265134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6NYC1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.