KIF13B Polyclonal antibody, PBS Only

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• KIF13B Polyclonal antibody, PBS Only (30141-1-PBS)

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  WB analysis using 30141-1-AP (same clone as 30141-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30141-1-AP (KIF13B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30141-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 30141-1-AP (same clone as 30141-1-PBS)

  WB result of KIF13B antibody (30141-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF13B transfected HepG2 cells. This data was developed using the same antibody clone with 30141-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 30141-1-AP (same clone as 30141-1-PBS)

  IP result of anti-KIF13B (IP:30141-1-AP, 4ug; Detection:30141-1-AP 1:800) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 30141-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30141-1-AP (same clone as 30141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30141-1-AP (KIF13B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30141-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30141-1-AP (same clone as 30141-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30141-1-AP (KIF13B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30141-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

KIF13B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 30141-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

GAKIN, KIAA0639, kinesin family member 13B

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Product Information

30141-1-PBS targets KIF13B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30876

  Product name: Recombinant human KIF13B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1648-1826 aa of NM_015254

  Sequence: VRRVRASELRSFSRMLAGDPGCSPGAEGNAPAPGAGGQALASDSEEADEVPEWLREGEFVTVGAHKTGVVRYVGPADFQEGTWVGVELDLPSGKNDGSIGGKQYFRCNPGYGLLVRPSRVRRATGPVRRRSTGLRLGAPEARRSATLSGSATNLASLTAALAKADRSHKNPENRKSWAS

• Full Name: kinesin family member 13B
• Calculated Molecular Weight: 203 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: NM_015254
• Gene Symbol: KIF13B
• Gene ID (NCBI): 23303
• RRID: AB_3086245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQT8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KIF13B, Also known as GAKIN, is a 250-kD phosphoprotein. KIF13B contains a consensus ATP/GTP-binding motif within its N-terminal motor domain; a stalk domain containing several short alpha-helical segments; and a CAP-gly motif in its C terminus characteristic of microtubule-based cytoskeleton components(PMID: 10859302).