CoraLite® Plus 488-conjugated KLK11 Monoclonal antibody

KLK11 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E2B4

Cat no : CL488-67427

Synonyms

Hippostasin, hK11, Kallikrein 11, KLK11, PRSS20, Serine protease 20, TLSP, Trypsin like protease

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Validation Data Gallery

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  IF Staining of human breast cancer using CL488-67427

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 KLK11 antibody (CL488-67427, Clone: 1E2B4 ) at dilution of 1:200.

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  IF Staining of human breast cancer using CL488-67427

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 KLK11 antibody (CL488-67427, Clone: 1E2B4 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67427 targets KLK11 in IF applications and shows reactivity with Human, Pig, Rat samples.

• Tested Reactivity: Human, Pig, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLK11 fusion protein Ag29895
• Full Name: kallikrein-related peptidase 11
• Calculated Molecular Weight: 250 aa, 27 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC022068
• Gene Symbol: KLK11
• Gene ID (NCBI): 11012
• RRID: AB_2919469
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.