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Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14965-1-AP

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19



Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, HepG2 cells, BxPC-3 cells
Positive IHC detected inhuman colon tissue, human lung cancer tissue, human liver cancer tissue, human cervical cancer tissue, rat colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, A431 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:6400
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

14965-1-AP targets Cytokeratin 19 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag6830
Full Name keratin 19
Calculated molecular weight 44 kDa
Observed molecular weight 40 kDa, 46 kDa
GenBank accession numberBC002539
Gene symbol KRT19
Gene ID (NCBI) 3880
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 14965-1-APDownload protocol
IHC protocol for Cytokeratin 19 antibody 14965-1-APDownload protocol
IF protocol for Cytokeratin 19 antibody 14965-1-APDownload protocol
FC protocol for Cytokeratin 19 antibody 14965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Am J Physiol Endocrinol Metab

Energy Metabolism Couples Hepatocyte Integrin-linked Kinase to Liver Glucoregulation and the Postabsorptive Response of Mice in an Age-dependent Manner.

Authors - Elijah Trefts
humanIHC

Nature

Syndecan 1 is a critical mediator of macropinocytosis in pancreatic cancer.

Authors - Wantong Yao
humanWB

Front Physiol

Multiscale and Multimodal Optical Imaging of the Ultrastructure of Human Liver Biopsies.

Authors - Cihang Kong
monkeyIF

Biochim Biophys Acta Mol Basis Dis

The zonula occludens protein family regulates the hepatic barrier system in the murine liver.

Authors - Masahiko Itoh
mouseIF

Front Cell Dev Biol

Volumetric imaging reveals VEGF-C-dependent formation of hepatic lymph vessels in mice

Authors - Stefanie Bobe
mouseIHC

Arch Toxicol

Galunisertib modifies the liver fibrotic composition in the Abcb4Ko mouse model.

Authors - Seddik Hammad