CoraLite®594-conjugated LCK Monoclonal antibody

LCK Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D10C2

Cat no : CL594-60162

Synonyms

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16

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Validation Data Gallery

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  FC experiment of A431 using CL594-60162

  1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human LCK (CL594-60162, Clone:2D10C2) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: A431 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60162 targets LCK in FC (Intra) applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LCK fusion protein Ag7694
• Full Name: lymphocyte-specific protein tyrosine kinase
• Calculated Molecular Weight: 539 aa, 56 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC013200
• Gene Symbol: LCK
• Gene ID (NCBI): 3932
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.