Tested Applications
| Positive IF-P detected in | rat brain tissue |
| Positive FC (Intra) detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67015 targets MAP2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11349 Product name: Recombinant human MAP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 213-559 aa of BC038857 Sequence: TSAGSTDRLPYSKSGNKDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVRILNKKIDFSKVQSRCGSKDNIKHSAGGGNVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL Predict reactive species |
| Full Name | microtubule-associated protein 2 |
| Calculated Molecular Weight | 200 kDa |
| GenBank Accession Number | BC038857 |
| Gene Symbol | MAP2 |
| Gene ID (NCBI) | 4133 |
| RRID | AB_2934512 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11137 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 MAP2 antibody CL488-67015 | Download protocol |
| IF protocol for CL Plus 488 MAP2 antibody CL488-67015 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAP2 Monoclonal antibody (1C3E6), catalog number CL488-67015, has 1 citation to date. It appears in Frontiers in Pharmacology (IF 5.4), published in 2026. The cited study investigates TLR3 activation in microglia-containing cerebral organoids and its induction of antiviral factors against HIV-1 infection, placing the research at the intersection of neuroimmunology and virology. The antibody was used in a human species context.
| Species | Application | Title |
|---|---|---|
Front Pharmacol TLR3 activation of microglia-containing cerebral organoid induces antiviral factors against HIV-1 infection. |
