MAP2 Monoclonal antibody

MAP2 Monoclonal Antibody for IHC,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1C3E6

Cat no : 67015-1-Ig

Synonyms

MAP 2, MAP2, MAP2A, MAP2B, MAP2C



Tested Applications

Positive IHC detected inmouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

67015-1-Ig targets MAP2 in IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAP2 fusion protein Ag11349
Full Name microtubule-associated protein 2
Calculated molecular weight 200 kDa
GenBank accession numberBC038857
Gene symbol MAP2
Gene ID (NCBI) 4133
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.

Protocols

Product Specific Protocols
IHC protocol for MAP2 antibody 67015-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Stem Cell

In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.

Authors - Wenjiao Tai
  • KO Validated
ratIF

Front Neurosci

Tetramethylpyrazine Alleviates Behavioral and Psychological Symptoms of Dementia Through Facilitating Hippocampal Synaptic Plasticity in Rats With Chronic Cerebral Hypoperfusion.

Authors - Zihu Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical cells went trough ICC and the MAP2 antibody at the 1/1000 dilution was used overnight at 4°C.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human primary cortical cells
MAP2 Antibody Immunohistochemistry validation (1/1000 dilution) in Human primary cortical cells (Cat no:67015-1-Ig)