Validation Data Gallery
|Positive WB detected in
|HepG2 cells, HL-60 cells, HEK-293 cells, mouse liver tissue, rat liver tissue
|Positive IP detected in
|Positive IHC detected in
|human renal cell carcinoma tissue, human liver cancer tissue, rat heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:10-1:100
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15904-1-AP targets MDH1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|MDH1 fusion protein Ag8744
|malate dehydrogenase 1, NAD (soluble)
|Calculated molecular weight
|334 aa, 36 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)
|Product Specific Protocols
|WB protocol for MDH1 antibody 15904-1-AP
|IHC protocol for MDH1 antibody 15904-1-AP
|IF protocol for MDH1 antibody 15904-1-AP
|IP protocol for MDH1 antibody 15904-1-AP
|Click here to view our Standard Protocols
An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis.
The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.
MDH1-mediated malate-aspartate NADH shuttle maintains the activity levels of fetal liver hematopoietic stem cells.
J Exp Med
Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.
cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.
Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.