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MDH1 Polyclonal antibody

MDH1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15904-1-AP

Synonyms

Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, human spleen tissue, HL-60 cells, HEK-293 cells, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman renal cell carcinoma tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15904-1-AP targets MDH1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MDH1 fusion protein Ag8744
Full Name malate dehydrogenase 1, NAD (soluble)
Calculated molecular weight 334 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC001484
Gene symbol MDH1
Gene ID (NCBI) 4190
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)

Protocols

Product Specific Protocols
WB protocol for MDH1 antibody 15904-1-APDownload protocol
IHC protocol for MDH1 antibody 15904-1-APDownload protocol
IF protocol for MDH1 antibody 15904-1-APDownload protocol
IP protocol for MDH1 antibody 15904-1-APDownload protocol
FC protocol for MDH1 antibody 15904-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Ethnopharmacol

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Authors - Li Wu
mouseWB

Reproduction

Nicotine reduces Sord expression and tyrosine phosphorylation in capacitated sperm.

Authors - Jingbo Dai
ratWB

J Proteome Res

iTRAQ-based proteomic analysis reveals recovery of impaired mitochondrial function in ischemic myocardium by Shenmai formula.

Authors - Yi Wang
humanWB

Nat Commun

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Authors - Xiaoyu Ma
humanWB

Cell Res

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Authors - Linchong Sun
mouseWB

Blood

MDH1-mediated malate-aspartate NADH shuttle maintains the activity levels of fetal liver hematopoietic stem cells.

Authors - Hao Gu