MEF2D Recombinant antibody

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  WB analysis using 85801-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85801-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85801-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85801-5-RR (MEF2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 85801-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEF2D antibody (85801-5-RR, Clone: 250068B7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MEF2D Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85801-5-RR

Clone No.250068B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Myocyte-specific enhancer factor 2D

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Tested Applications

• Positive WB detected in: HeLa cells, THP-1 cells, K-562 cells, U2OS cells, C2C12 cells, C6 cells, U266 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85801-5-RR targets MEF2D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MEF2D fusion protein Ag5700
• Full Name: myocyte enhancer factor 2D
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC040949
• Gene Symbol: MEF2D
• Gene ID (NCBI): 4209
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14814
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEF2D, also named as Myocyte-specific enhancer factor 2D, is a 521 amino acid protein, which belongs to the MEF2 family. MEF2D is expressed in t in myotubes and also in undifferentiated myoblasts. MEF2D as a transcriptional activator which binds specifically to the MEF2 element, found in numerous muscle-specific and stress-induced genes. MEF2D mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. The calculated molecular weight of MEF2D is 55 kDa, but Phosphorylated MEF2D is 60 kDa.