HRP-conjugated MTAP Monoclonal antibody

MTAP Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB

Conjugate

HRP

CloneNo.

2B1G6

Cat no : HRP-66706

Synonyms

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

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Validation Data Gallery

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  WB analysis of NIH/3T3 using HRP-66706

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with HRP-66706 (MTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

HRP-66706 targets MTAP in WB applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTAP fusion protein Ag17954
• Full Name: methylthioadenosine phosphorylase
• Calculated Molecular Weight: 283 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC018625
• Gene Symbol: MTAP
• Gene ID (NCBI): 4507
• RRID: AB_2883844
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.