MTHFD1L Monoclonal antibody

MTHFD1L Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Rabbit, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1F2

Cat no : 68321-1-Ig

Synonyms

dJ292B18.2, DKFZp586G1517, FLJ21145, FTHFSDC1, MTC1THFS, MTHFD1L

---

Validation Data Gallery

• 

  WB analysis using 68321-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 68321-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  IHC staining of mouse liver using 68321-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 68321-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: U2OS cells, rabbit testis tissue, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, rat testis tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68321-1-Ig targets MTHFD1L in WB, IHC, ELISA applications and shows reactivity with Human, Rat, Rabbit, Mouse samples.

• Tested Reactivity: Human, Rat, Rabbit, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTHFD1L fusion protein Ag9061
• Full Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
• Calculated Molecular Weight: 978 aa, 106 kDa
• Observed Molecular Weight: 106 kDa
• GenBank Accession Number: BC017477
• Gene Symbol: MTHFD1L
• Gene ID (NCBI): 25902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.