MYBPC3 Monoclonal antibody

MYBPC3 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C8F8

Cat no : 67608-1-Ig

Synonyms

Cardiac MyBP C, CMH4, DKFZp779E1762, FHC, MYBP C, MYBPC3

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Validation Data Gallery

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  WB analysis of mouse heart using 67608-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of H9C2 using 67608-1-Ig

  H9C2 cells were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit heart using 67608-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67608-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67608-1-Ig (MYBPC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 67608-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 67608-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67608-1-Ig (MYBPC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat heart using 67608-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using MYBPC3 antibody (67608-1-Ig, Clone: 3C8F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, H9C2 cells, rabbit heart tissue, pig heart tissue
• Positive IHC detected in: human heart tissue, rat heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67608-1-Ig targets MYBPC3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYBPC3 fusion protein Ag15848
• Full Name: myosin binding protein C, cardiac
• Calculated Molecular Weight: 1274 aa, 141 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC151211
• Gene Symbol: MYBPC3
• Gene ID (NCBI): 4607
• RRID: AB_2882814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4).