SMMHC Polyclonal antibody

SMMHC Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 21404-1-AP

Synonyms

MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain



Tested Applications

Positive WB detected inmouse uterus tissue
Positive IHC detected inhuman colon tissue, human skeletal muscle tissue, rat bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Positive FC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21404-1-AP is SMMHC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMMHC fusion protein Ag16114
Full Name myosin, heavy chain 11, smooth muscle
Calculated molecular weight 1979 aa, 228 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC104906
Gene symbol MYH11
Gene ID (NCBI) 4629
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.

Protocols

Product Specific Protocols
WB protocol for SMMHC antibody 21404-1-APDownload protocol
IHC protocol for SMMHC antibody 21404-1-APDownload protocol
IF protocol for SMMHC antibody 21404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease.

Authors - Leonardo Elia
mouseWB

Theranostics

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Authors - Gao-Shan Yang
mouseIF

Elife

Coronary blood vessels from distinct origins converge to equivalent states during mouse and human development.

Authors - Ragini Phansalkar
human,ratWB

Arterioscler Thromb Vasc Biol

The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling.

Authors - Frances L Jourd'heuil
mouseWB

Sci Rep

Oral chromium picolinate impedes hyperglycemia-induced atherosclerosis and inhibits proatherogenic protein TSP-1 expression in STZ-induced type 1 diabetic ApoE(-/-) mice.

Authors - Rituparna Ganguly
humanWB

Front Cell Dev Biol

Vascular Adhesion Protein-1 Determines the Cellular Properties of Endometrial Pericytes.

Authors - Seley Gharanei