MYH6 Polyclonal antibody, PBS Only

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• MYH6 Polyclonal antibody, PBS Only (22281-1-PBS)

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  WB analysis using 22281-1-AP (same clone as 22281-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22281-1-AP (MYH6 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  IP result of anti-MYH6 (IP:22281-1-AP, 5ug; Detection:22281-1-AP 1:300) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using MYH6 antibody (22281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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  IF Staining of HSkMC using 22281-1-AP (same clone as 22281-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HSkMC cells using MYH6 antibody (22281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MYH6 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 22281-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Myosin 6, MYHCA, MyHC-alpha, Myosin heavy chain 6, Myosin-6

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Product Information

22281-1-PBS targets MYH6 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin, heavy chain 6, cardiac muscle, alpha
• Calculated Molecular Weight: 224 kDa
• Observed Molecular Weight: 200-220 kDa
• GenBank Accession Number: NM_002471
• Gene Symbol: MYH6
• Gene ID (NCBI): 4624
• RRID: AB_2736822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13533
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)