NCALD Monoclonal antibody

NCALD Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

6B4E10

Cat no : 66088-1-Ig

Synonyms

NCALD, neurocalcin delta, neurocalcin δ



Tested Applications

Positive WB detected infetal human brain tissue, pig brain tissue, pig cerebellum tissue, human brain tissue
Positive IHC detected inhuman cerebellum tissue, mouse cerebellum tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3200
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66088-1-Ig is NCALD Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NCALD fusion protein Ag18760
Full Name neurocalcin delta
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC036098
Gene symbol NCALD
Gene ID (NCBI) 83988
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.

Protocols

Product Specific Protocols
WB protocol for NCALD antibody 66088-1-IgDownload protocol
IHC protocol for NCALD antibody 66088-1-IgDownload protocol
IF protocol for NCALD antibody 66088-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols