NCALD Monoclonal antibody
NCALD Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, pig, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
6B4E10
Cat no : 66088-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue, human brain tissue, pig cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue |
Positive IHC detected in | human cerebellum tissue, mouse cerebellum tissue, human testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3200 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
66088-1-Ig targets NCALD in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
Tested Reactivity | human, mouse, pig, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | NCALD fusion protein Ag18760 |
Full Name | neurocalcin delta |
Calculated molecular weight | 193 aa, 22 kDa |
Observed molecular weight | 22 kDa |
GenBank accession number | BC036098 |
Gene symbol | NCALD |
Gene ID (NCBI) | 83988 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.
Protocols
Product Specific Protocols | |
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WB protocol for NCALD antibody 66088-1-Ig | Download protocol |
IHC protocol for NCALD antibody 66088-1-Ig | Download protocol |
IF protocol for NCALD antibody 66088-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |