NCALD Monoclonal antibody

NCALD Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a


human, mouse, pig, rat







Cat no : 66088-1-Ig


NCALD, neurocalcin delta, neurocalcin δ

Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, pig cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inhuman cerebellum tissue, mouse cerebellum tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3200
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66088-1-Ig targets NCALD in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NCALD fusion protein Ag18760
Full Name neurocalcin delta
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC036098
Gene symbol NCALD
Gene ID (NCBI) 83988
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.


Product Specific Protocols
WB protocol for NCALD antibody 66088-1-IgDownload protocol
IHC protocol for NCALD antibody 66088-1-IgDownload protocol
IF protocol for NCALD antibody 66088-1-IgDownload protocol
Standard Protocols
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