CoraLite® Plus 488-conjugated NCALD Monoclonal antibody

NCALD Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6B4E10

Cat no : CL488-66088

Synonyms

NCALD, neurocalcin delta



Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66088 is NCALD Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NCALD fusion protein Ag18760
Full Name neurocalcin delta
Calculated molecular weight 193 aa, 22 kDa
GenBank accession numberBC036098
Gene symbol NCALD
Gene ID (NCBI) 83988
RRIDAB_2919285
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NCALD antibody CL488-66088Download protocol
Standard Protocols
Click here to view our Standard Protocols