CoraLite®594-conjugated NCALD Monoclonal antibody

NCALD Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

6B4E10

Cat no : CL594-66088

Synonyms

NCALD, neurocalcin delta



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66088-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66088 is NCALD Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NCALD fusion protein Ag18760
Full Name neurocalcin delta
Calculated molecular weight 193 aa, 22 kDa
GenBank accession numberBC036098
Gene symbol NCALD
Gene ID (NCBI) 83988
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.

Protocols

Product Specific Protocols
IF protocol for CL594 NCALD antibody CL594-66088Download protocol
Standard Protocols
Click here to view our Standard Protocols