NCAPG Polyclonal antibody, PBS Only

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• NCAPG Polyclonal antibody, PBS Only (24563-1-PBS)

• 

  WB analysis using 24563-1-AP (same clone as 24563-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 24563-1-AP (same clone as 24563-1-PBS)

  WB result of NCAPG antibody (24563-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPG transfected HEK-293 cells. This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 24563-1-AP (same clone as 24563-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 24563-1-AP (same clone as 24563-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24563-1-AP (same clone as 24563-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 24563-1-AP (same clone as 24563-1-PBS)

  IP result of anti-NCAPG (IP:24563-1-AP, 4ug; Detection:24563-1-AP 1:8000) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 24563-1-AP (same clone as 24563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 24563-1-AP (same clone as 24563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 24563-1-AP (same clone as 24563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 24563-1-AP (same clone as 24563-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 24563-1-AP (same clone as 24563-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NCAPG antibody (24563-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 24563-1-AP (same clone as 24563-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NCAPG antibody (24563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24563-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NCAPG Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 24563-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CAPG, CHCG, Chromosome-associated protein G, Condensin complex subunit 3, Condensin subunit CAP G

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Product Information

24563-1-PBS targets NCAPG in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19926

  Product name: Recombinant human NCAPG protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 664-1015 aa of BC068467

  Sequence: TLHCEGTEINSDDEQESKEVEETATAKNVLKLLSDFLDSEVSELRTGAAEGLAKLMFSGLLVSSRILSRLILLWYNPVTEEDVQLRHCLGVFFPVFAYASRTNQECFEEAFLPTLQTLANAPASSPLAEIDITNVAELLVDLTRPSGLNPQAKTSQDYQALTVHDNLAMKICNEILTSPCSPEIRVYTKALSSLELSSHLAKDLLVLLNEILEQVKDRTCLRALEKIKIQLEKGNKEFGDQAEAAQDATLTTTTFQNEDEKNKEVYMTPLRGVKATQASKSTQLKTNRGQRKVTVSARTNRRCQTAEADSESDHEVPEPESEMKMRLPRRAKTAALEKSKLNLAQFLNEDLS

• Full Name: non-SMC condensin I complex, subunit G
• Calculated Molecular Weight: 1015 aa, 114 kDa
• Observed Molecular Weight: 131 kDa
• GenBank Accession Number: BC068467
• Gene Symbol: NCAPG
• Gene ID (NCBI): 64151
• RRID: AB_2879610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BPX3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.