NEU1 Monoclonal antibody

NEU1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F6D1

Cat no : 67032-1-Ig

Synonyms

Acetylneuraminyl hydrolase, FLJ93471, G9 sialidase, Lysosomal sialidase, N acetyl alpha neuraminidase 1, NANH, NEU, NEU1, NEURAMINIDASE 1, SIAL1, Sialidase 1



Tested Applications

Positive WB detected inPC-12 cells, rat pancreas tissue, pig liver tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67032-1-Ig targets NEU1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Rat, Pig samples.

Tested Reactivity Human, Rat, Pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEU1 fusion protein Ag27945
Full Name sialidase 1 (lysosomal sialidase)
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC011900
Gene Symbol NEU1
Gene ID (NCBI) 4758
RRIDAB_2882347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEU1 (lysosomal sialidase) encodes a kind of lysosomal enzyme that could cleave terminal silic acids from glycoproteins or glycolipids to regulate various biological processes by conformational change (PMID:28130415). In the lysosome, NEU1 is one member of a heterotrimeric complex, the others are beta-galactosidase and cathepsin A. The NEU1 is widely expressed in mammalian tissues and involved in lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells (PMID:19075514). NUE1 also expresses on the plasma membrane where it modulates several signalling molecules about inflamation, exocytosis, phagocytosis, cell adhesion and proliferation (PMID:21928149). Addition, it is reported that mutation in NEU1 of human could lead to sialidosis (PMID:14517945).

Protocols

Product Specific Protocols
WB protocol for NEU1 antibody 67032-1-IgDownload protocol
IHC protocol for NEU1 antibody 67032-1-IgDownload protocol
IF protocol for NEU1 antibody 67032-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur Rev Med Pharmacol Sci

Long non-coding RNA TOB1-AS1 modulates cell proliferation, apoptosis, migration and invasion through miR-23a/NEU1 axis via Wnt/b-catenin pathway in gastric cancer.

Authors - K Jiang
humanIF

Br J Pharmacol

Neuraminidase is a host-directed approach to regulate neutrophil responses in sepsis and COVID-19

Authors - Rodrigo de Oliveira Formiga
mouse,humanWB,IF,IP

Nat Commun

Neuraminidase 1 promotes renal fibrosis development in male mice

Authors - Qian-Qian Chen