NOX2 Recombinant antibody

NOX2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B17

Cat no : 82872-1-RR

Synonyms

CGD, CGD91 phox, CYBB, Cytochrome b 245 heavy chain, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, GP91 1, GP91 PHOX, GP91PHOX, NADPH oxidase 2, NOX2, p22 phagocyte B cytochrome, p91 PHOX

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Validation Data Gallery

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  WB analysis using 82872-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82872-1-RR (CYBB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse spleen tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82872-1-RR targets NOX2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NOX2 fusion protein Ag4402
• Full Name: cytochrome b-245, beta polypeptide
• Calculated Molecular Weight: 577 aa, 65 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC032720
• Gene Symbol: CYBB
• Gene ID (NCBI): 1536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa(PMID: 17587483) to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781).