NeuN Polyclonal antibody

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  WB analysis of mouse brain using 23060-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 23060-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 23060-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 23060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23060-1-AP (NeuN Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse brain using 23060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23060-1-AP (NeuN Antibody) at dilution of 1:200 (under 40x lens).

NeuN antibodies are widely used as neuronal markers to identify and quantify mature neurons in brain and spinal cord tissue in neuroscience research. Proteintech has an extensive validated NeuN antibodies for WB, IHC, ELISA.

Cat No. 23060-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: mouse brain tissue, 3T3-L1 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 8 publications below

Product Information

23060-1-AP targets NeuN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, hamster, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NeuN fusion protein Ag19347
• Full Name: hexaribonucleotide binding protein 3
• Observed Molecular Weight: 38-50 kDa
• GenBank Accession Number: BC093713
• Gene Symbol: NeuN
• Gene ID (NCBI): 146713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A6NFN3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NeuN, also named as FOX3 and RBFOX3, is neuronal-specific nuclear protein with MW 38-50kd(~48kd). It is one of a family of 3 mammalian Fox homologues. FOX3(Or NeuN) functions in RNA-binding protein that regulates alternative splicing events.

Publications

Species	Application	Title
goat	IF	Cell Cycle; Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.; Authors - Zhimin Wang
hamster	IHC	Neurotherapeutics; PMID: 28083805; Authors - Syed Zahid Ali Shah
rat	IF	Exp Cell Res; CEBPD aggravates apoptosis and oxidative stress of neuron after ischemic stroke by Nrf2/HO-1 pathway; Authors - Nan Chen
rat	WB,IF	Neurochem Int; Tert-butylhydroquinone post-treatment attenuates neonatal hypoxic-ischemic brain damage in rats.; Authors - Juan Zhang
mouse	IF	J Stroke Cerebrovasc Dis; An Increase of Sigma-1 Receptor in the Penumbra Neuron after Acute Ischemic Stroke.; Authors - Xiaotian Zhang
human	IHC	Pediatr Surg Int; Pathological changes of interstitial cells of Cajal and ganglion cells in the segment of resected bowel in Hirschsprung's disease.; Authors - Xuyong Chen