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Tested Applications
| Positive WB detected in | mouse brain tissue, 3T3-L1 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 4 publications below |
| IF | See 8 publications below |
Product Information
23060-1-AP targets NeuN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, hamster, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NeuN fusion protein Ag19347 Predict reactive species |
| Full Name | hexaribonucleotide binding protein 3 |
| Observed Molecular Weight | 38-50 kDa |
| GenBank Accession Number | BC093713 |
| Gene Symbol | NeuN |
| Gene ID (NCBI) | 146713 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A6NFN3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NeuN, also named as FOX3 and RBFOX3, is neuronal-specific nuclear protein with MW 38-50kd(~48kd). It is one of a family of 3 mammalian Fox homologues. FOX3(Or NeuN) functions in RNA-binding protein that regulates alternative splicing events.
Publications
| Species | Application | Title |
|---|---|---|
Cell Cycle Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells. | ||
Exp Cell Res CEBPD aggravates apoptosis and oxidative stress of neuron after ischemic stroke by Nrf2/HO-1 pathway | ||
Neurochem Int Tert-butylhydroquinone post-treatment attenuates neonatal hypoxic-ischemic brain damage in rats. | ||
J Stroke Cerebrovasc Dis An Increase of Sigma-1 Receptor in the Penumbra Neuron after Acute Ischemic Stroke. | ||
Pediatr Surg Int Pathological changes of interstitial cells of Cajal and ganglion cells in the segment of resected bowel in Hirschsprung's disease. |
