mouse lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 85030-1-RR
rat lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
rat lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary cancer using 85030-1-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85030-1-RR (PDE5A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85030-1-RR (PDE5A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SKOV-3 using 85030-1-RR
Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDE5A antibody (85030-1-RR, Clone: 242597B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDE5A antibody (85030-1-RR, Clone: 242597B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HEK-293 using 85030-1-RR
1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PDE5A Recombinant antibody (85030-1-RR, Clone:242597B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PDE5A Recombinant antibody (85030-1-RR, Clone:242597B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Affinity and Kinetic Characterization of 85030-1-RR
Biolayer interferometry (BLl) kinetic assays of 85030-1-RR against Human PDE5A were performed. The affinity constant is 1.07 nM.
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human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
SKOV-3 cells
Positive FC (Intra) detected in
HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
85030-1-RR targets PDE5A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
PDE5A (cGMP-binding cGMP-specific phosphodiesterase) plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Through alternative splicing, the PDE5A gene produces three proteins (PDE5A1, PDE5A2, and PDE5A3) that differ in their N termini and range in mass from 89 to 105 kDa. (PMID: 25704994; 21215707). A second 70 kDa band was consistently observed in heart tissue that either reflected a splice variant or proteolytic fragment of PDE5A. (PMID: 15576651).
mouse lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 85030-1-RR
rat lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary cancer using 85030-1-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85030-1-RR (PDE5A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of SKOV-3 using 85030-1-RR
Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDE5A antibody (85030-1-RR, Clone: 242597B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC (INTRA) Figures
FC experiment of HEK-293 using 85030-1-RR
1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PDE5A Recombinant antibody (85030-1-RR, Clone:242597B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
AFFINITY Figures
Affinity and Kinetic Characterization of 85030-1-RR
Biolayer interferometry (BLl) kinetic assays of 85030-1-RR against Human PDE5A were performed. The affinity constant is 1.07 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
PDE5A Recombinant antibody
Catalog Number
85030-1-RR
Citations
-
Dilutions
WB : 1:2000-1:10000 IHC : 1:300-1:1200 IF/ICC : 1:200-1:800 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
