POP1 Polyclonal antibody, PBS Only

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  WB analysis using 12029-1-AP (same clone as 12029-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12029-1-AP (POP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

  WB result of POP1 antibody (12029-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POP1 transfected HeLa cells. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

  IP result of anti-POP1 (IP:12029-1-AP, 4ug; Detection:12029-1-AP 1:8000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12029-1-AP (same clone as 12029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12029-1-AP (POP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12029-1-AP (same clone as 12029-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POP1 antibody (12029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

POP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12029-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

POP 1

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Product Information

12029-1-PBS targets POP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2660

  Product name: Recombinant human POP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC011529

  Sequence: MSNAKERKHAKKMRNQPTNVTLSSGFVADRGVKHHSGGEKPFQAQKQEPHPGTSRQRQTRVNPHSLPDPEVNEQSSSKGMFRKKGGWKAGPEGTSQEIPKYITASTFAQARAAEISAMLKAVTQKSSNSLVFQTLPRHMRRRAMSHNVKRLPRRLQEIAQKEAEKAVHQKKEHSKNKCHKARRCHMNRTLEFNRRQKKNIWLETHIWHAKRFHMVKKWGYCLGERPTVKSHRACYRAMTNRCLLQDLSYYCCLELKGKEEEILKALSGMCNIDTGLTFAAVHCLSGKRQGSLVLYRVNKYPREMLGPVTFIWKSQRTPGDPSESRQLWIWLHPTLKQDILEEIKAACQCV

• Full Name: processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
• Calculated Molecular Weight: 1024 aa, 115 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC011529
• Gene Symbol: POP1
• Gene ID (NCBI): 10940
• RRID: AB_2166477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99575
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.