CoraLite® Plus 488-conjugated PSMD3 Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-84928-2

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PSMD3 antibody (CL488-84928-2, Clone: 242470D4 ) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of A549 using CL488-84928-2

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 PSMD3 antibody (CL488-84928-2, Clone: 242470D4 ) at dilution of 1:200, CL594-Phalloidin (red).

PSMD3 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84928-2

Clone No.242470D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

242470D4, 26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84928-2 targets PSMD3 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2684

  Product name: Recombinant human PSMD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 235-534 aa of BC012302

  Sequence: LRTATLRHDADGQATLLNLLLRNYLHYSLYDQAEKLVSKSVFPEQANNNEWARYLYYTGRIKAIQLEYSVARRTMTNALRKAPQHTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQPSLKRSLMPYFLLTQAVRTGNLAKFNQVLDQFGEKFQADGTYTLIIRLRHNVIKTGVRMISLSYSRISLADIAQKLQLDSPEDAEFIVAKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMSVKAMRFPPKSYNKDLESAEERREREQQDLEFAKEMAEDDDDSFP

• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
• Calculated Molecular Weight: 534 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC012302
• Gene Symbol: PSMD3
• Gene ID (NCBI): 5709
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43242
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).