PTGER4 Monoclonal antibody, PBS Only

PTGER4 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A2A12

Cat no : 66921-1-PBS

Synonyms

EP4, EP4R, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor, PTGER2, PTGER4

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Validation Data Gallery

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  WB analysis of pig brain using 66921-1-Ig (same clone as 66921-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66921-1-Ig (same clone as 66921-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66921-1-Ig (same clone as 66921-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66921-1-Ig (same clone as 66921-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PTGER4 antibody (66921-1-Ig, Clone: 4A2A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PTGER4 antibody (66921-1-Ig, Clone: 4A2A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66921-1-Ig (same clone as 66921-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PTGER4 (66921-1-Ig, Clone:4A2A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66921-1-PBS targets PTGER4 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTGER4 fusion protein Ag19262
• Full Name: prostaglandin E receptor 4 (subtype EP4)
• Calculated Molecular Weight: 488 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC101534
• Gene Symbol: PTGER4
• Gene ID (NCBI): 5734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.