PTGER4 Monoclonal antibody

PTGER4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

4A2A12

Cat no : 66921-1-Ig

Synonyms

EP4R, EP4, 4A2A12

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Validation Data Gallery

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  WB analysis of pig brain using 66921-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66921-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66921-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66921-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66921-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 66921-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 66921-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66921-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66921-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 66921-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PTGER4 antibody (66921-1-Ig, Clone: 4A2A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66921-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PTGER4 antibody (66921-1-Ig, Clone: 4A2A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66921-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PTGER4 (66921-1-Ig, Clone:4A2A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: pig brain tissue, rat brain tissue, mouse brain tissue, Jurkat cells, K-562 cells
• Positive IHC detected in: human prostate hyperplasia tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

66921-1-Ig targets PTGER4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTGER4 fusion protein Ag19262
• Full Name: prostaglandin E receptor 4 (subtype EP4)
• Calculated Molecular Weight: 488 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC101534
• Gene Symbol: PTGER4
• Gene ID (NCBI): 5734
• RRID: AB_2882248
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; m6A demethylase ALKBH5 is required for antibacterial innate defense by intrinsic motivation of neutrophil migration.; Authors - Yang Liu
human,mouse	WB	J Mol Med (Berl); Vav2 promotes ductus arteriosus anatomic closure via the remodeling of smooth muscle cells by Rac1 activation; Authors - Yinghui Chen
mouse	IHC	Nat Commun; Post-resolution macrophages shape long-term tissue immunity and integrity in a mouse model of pneumococcal pneumonia; Authors - Karen T Feehan
human,mouse	WB	Cell Metab; m6A mRNA methylation in brown fat regulates systemic insulin sensitivity via an inter-organ prostaglandin signaling axis independent of UCP1; Authors - Ling Xiao