PTGES3 Monoclonal antibody

PTGES3 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

3C11D11

Cat no : 67736-1-Ig

Synonyms

cPGES, Hsp90 co chaperone, P23, Prostaglandin E synthase 3, PTGES3, TEBP, Telomerase binding protein p23

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Validation Data Gallery

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  WB analysis using 67736-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67736-1-Ig (PTGES3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67736-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67736-1-Ig (PTGES3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67736-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67736-1-Ig (PTGES3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67736-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES3 antibody (67736-1-Ig, Clone: 3C11D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67736-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES3 antibody (67736-1-Ig, Clone: 3C11D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, PC-3 cells, HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

67736-1-Ig targets PTGES3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

• Tested Reactivity: Human, mouse, pig, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTGES3 fusion protein Ag7870
• Full Name: prostaglandin E synthase 3 (cytosolic)
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003005
• Gene Symbol: PTGES3
• Gene ID (NCBI): 10728
• RRID: AB_2918505
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).

Publications

Species	Application	Title
human	WB	Int Immunopharmacol; High expression of PTGES3 is an independent predictive poor prognostic biomarker and correlates with immune infiltrates in lung adenocarcinoma.; Authors - Pengxiang Gao
human	WB	Cell Death Discov; METTL9-SLC7A11 axis promotes hepatocellular carcinoma progression through ferroptosis inhibition; Authors - Fangfang Bi
human	WB	J Transl Med; The integration of multidisciplinary approaches revealed PTGES3 as a novel drug target for breast cancer treatment; Authors - Qinan Yin