PTPN6 Polyclonal antibody

PTPN6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 24546-1-AP

Synonyms

HCP, HCPH, HPTP1C, PTP 1C, PTP1C, PTPN6, SH PTP1, SHP 1, SHP 1L, SHP1



Tested Applications

Positive WB detected inJurkat cells, Raji cells, A431 cells, HuH-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, A431 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24546-1-AP is PTPN6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN6 fusion protein Ag21415
Full Name protein tyrosine phosphatase, non-receptor type 6
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC002523
Gene symbol PTPN6
Gene ID (NCBI) 5777
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN6(Tyrosine-protein phosphatase non-receptor type 6) is also named as HCP, PTP1C and belongs to the protein-tyrosine phosphatase family. It regulates muscle INS action in a cell-autonomous manner, further suggesting that the PTPase negatively modulates INS action through down-regulation of both INS signaling to AKT1 and SLC2A4 translocation, as well as SLC2A4 expression(PMID:21952243). It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PTPN6 antibody 24546-1-APDownload protocol
IHC protocol for PTPN6 antibody 24546-1-APDownload protocol
IF protocol for PTPN6 antibody 24546-1-APDownload protocol
IP protocol for PTPN6 antibody 24546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Elife

Molecular features underlying differential SHP1/SHP2 binding of immune checkpoint receptors.

Authors - Xiaozheng Xu
humanWB

Oncotarget

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Authors - Ighodaro Igbe
human,mouseWB

Front Immunol

Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response.

Authors - Yafeng Dou
  • KD Validated
humanWB

Int J Biol Sci

PMID: 29805309

Authors - Linna Xie
humanWB

Oxid Med Cell Longev

Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.

Authors - Amara Maryam
humanWB

J Biol Chem

A thiazole-derived oridonin analog exhibits antitumor activity by directly and allosterically inhibiting STAT3.

Authors - Xiaofei Shen