PTPN6 Polyclonal antibody

PTPN6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 24546-1-AP

Synonyms

HCP, HCPH, HPTP1C, PTP 1C, PTP1C, PTPN6, SH PTP1, SHP 1, SHP 1L, SHP1

---

Validation Data Gallery

• 

  WB analysis using 24546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24546-1-AP (PTPN6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 24546-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 24546-1-AP (SHP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24546-1-AP (SHP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 24546-1-AP

  IP result of anti-PTPN6 (IP:24546-1-AP, 4ug; Detection:24546-1-AP 1:600) with Jurkat cells lysate 3600ug.

• 

  IHC staining of human colon cancer using 24546-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24546-1-AP (PTPN6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 24546-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PTPN6 antibody (24546-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of MCF-7 using 24546-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PTPN6 antibody (24546-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 24546-1-AP

  Immunofluorescent analysis of A431 cells using 24546-1-AP (SHP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of MCF-7 using 24546-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PTPN6 (24546-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells, HuH-7 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, A431 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

24546-1-AP targets PTPN6 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPN6 fusion protein Ag21415
• Full Name: protein tyrosine phosphatase, non-receptor type 6
• Calculated Molecular Weight: 597 aa, 68 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC002523
• Gene Symbol: PTPN6
• Gene ID (NCBI): 5777
• RRID: AB_2879600
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN6(Tyrosine-protein phosphatase non-receptor type 6) is also named as HCP, PTP1C and belongs to the protein-tyrosine phosphatase family. It regulates muscle INS action in a cell-autonomous manner, further suggesting that the PTPase negatively modulates INS action through down-regulation of both INS signaling to AKT1 and SLC2A4 translocation, as well as SLC2A4 expression(PMID:21952243). It has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IF	Elife; Molecular features underlying differential SHP1/SHP2 binding of immune checkpoint receptors.; Authors - Xiaozheng Xu
human	WB	Oncotarget; Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.; Authors - Ighodaro Igbe
human,mouse	WB	Front Immunol; Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response.; Authors - Yafeng Dou ; KD Validated
human	WB	Int J Biol Sci; PMID: 29805309; Authors - Linna Xie
human	WB	Oxid Med Cell Longev; Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.; Authors - Amara Maryam
human	WB	J Biol Chem; A thiazole-derived oridonin analog exhibits antitumor activity by directly and allosterically inhibiting STAT3.; Authors - Xiaofei Shen