PTPN6 Polyclonal antibody
PTPN6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (1)
WB, IP, IHC, IF, FC, CoIP, ELISA
Cat no : 24546-1-AP
Validation Data Gallery
|Positive WB detected in||Jurkat cells, Raji cells, A431 cells, HuH-7 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, A431 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 11 publications below|
|IHC||See 1 publications below|
|IF||See 1 publications below|
|IP||See 1 publications below|
|CoIP||See 1 publications below|
The immunogen of 24546-1-AP is PTPN6 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PTPN6 fusion protein Ag21415|
|Full Name||protein tyrosine phosphatase, non-receptor type 6|
|Calculated molecular weight||597 aa, 68 kDa|
|Observed molecular weight||63 kDa|
|GenBank accession number||BC002523|
|Gene ID (NCBI)||5777|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PTPN6(Tyrosine-protein phosphatase non-receptor type 6) is also named as HCP, PTP1C and belongs to the protein-tyrosine phosphatase family. It regulates muscle INS action in a cell-autonomous manner, further suggesting that the PTPase negatively modulates INS action through down-regulation of both INS signaling to AKT1 and SLC2A4 translocation, as well as SLC2A4 expression(PMID:21952243). It has 4 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for PTPN6 antibody 24546-1-AP||Download protocol|
|IHC protocol for PTPN6 antibody 24546-1-AP||Download protocol|
|IF protocol for PTPN6 antibody 24546-1-AP||Download protocol|
|IP protocol for PTPN6 antibody 24546-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Molecular features underlying differential SHP1/SHP2 binding of immune checkpoint receptors.
Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.
Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response.
Int J Biol Sci
Oxid Med Cell Longev
Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.
J Biol Chem
A thiazole-derived oridonin analog exhibits antitumor activity by directly and allosterically inhibiting STAT3.