Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, human brain tissue, human lung tissue, HUVEC cells, U-251 cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, human cervical cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 ul suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11916-1-AP targets RABL3 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2531 Product name: Recombinant human RABL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-236 aa of BC020832 Sequence: MASLDRVKVLVLGDSGVGKSSLVHLLCQNQVLGNPSWTVGCSVDVRVHDYKEGTPEEKTCYIELWDVGGSVGSASSVKSTRAVFYNSVNGIIFVHDLTNKKSSQNLRRWSLEALNRDLVPTGVLVTNGDYDQEQFADNQIPLLVIGTKLDQIHETKRHEVLTTTAFLAEDFNPEEINLDCTNPRYLAAGSSNAVKLSRFFDKVIEKRYFLREGNQIPGFPDRKRFGAGTLKSLHYD Predict reactive species |
| Full Name | RAB, member of RAS oncogene family-like 3 |
| Calculated Molecular Weight | 236 aa, 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC020832 |
| Gene Symbol | RABL3 |
| Gene ID (NCBI) | 285282 |
| RRID | AB_2175960 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5HYI8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RABL3 antibody 11916-1-AP | Download protocol |
| IF protocol for RABL3 antibody 11916-1-AP | Download protocol |
| IHC protocol for RABL3 antibody 11916-1-AP | Download protocol |
| IP protocol for RABL3 antibody 11916-1-AP | Download protocol |
| WB protocol for RABL3 antibody 11916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RABL3 Polyclonal antibody (Cat# 11916-1-AP) has 2 total citations. They appear in Oncology Reports and Medical Science Monitor. Both studies used Western blot in human samples to examine RABL3's role in cancer. The research fields involve cancer biology, specifically RABL3's regulation of proliferation and motility in human cancer cells, and its association with poor survival in non-small cell lung cancer through repression of MAPK8/9/10-mediated autophagy.
| Species | Application | Title |
|---|---|---|
Oncol Rep Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells.
| ||
Med Sci Monit High Expression of Rab-like 3 (Rabl3) is Associated with Poor Survival of Patients with Non-Small Cell Lung Cancer via Repression of MAPK8/9/10-Mediated Autophagy.
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