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RAD50 Polyclonal antibody, PBS Only (29390-1-PBS)

Filter: AllWBIHCIF-PIF/ICC

WB analysis using 29390-1-AP (same clone as 29390-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29390-1-AP (RAD50 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 29390-1-AP (same clone as 29390-1-PBS)
WB result of RAD50 antibody (29390-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD50 transfected MCF-7 cells. This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 29390-1-AP (same clone as 29390-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 29390-1-AP (same clone as 29390-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.
IF Staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using RAD50 antibody (29390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 29390-1-AP (same clone as 29390-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 29390-1-AP (same clone as 29390-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

RAD50 Polyclonal antibody, PBS Only

Name: RAD50 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 29390-1-PBS

RAD50 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 29390-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

hRad50, RAD50 homolog (S. cerevisiae)

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

29390-1-PBS targets RAD50 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag29949 Product name: Recombinant human RAD50 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 275-425 aa of NM_005732 Sequence: LDSRKKQMEKDNSELEEKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCHRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQEHIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEGEAKTANQLMNDFAEKETLK Predict reactive species
Full Name	RAD50 homolog (S. cerevisiae)
Calculated Molecular Weight	154 kDa
Observed Molecular Weight	154 kDa
GenBank Accession Number	NM_005732
Gene Symbol	RAD50
Gene ID (NCBI)	10111
RRID	AB_2918289
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92878
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).