Tested Applications
| Positive WB detected in | HepG2 cells, mouse kidney tissue, mouse liver tissue, mouse colon tissue, mouse heart tissue, rat colon tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat eye tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15187-1-AP targets RBPMS in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7256 Product name: Recombinant human RBPMS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC003608 Sequence: MNNGGKAEKENTPSEANLQEEEVRTLFVSGLPLDIKPRELYLLFRPFKGYEGSLIKLTSKQPVGFVSFDSRSEAEAAKNALNGIRFDPEIPQTLRLEFAKANTKMAKNKLVGTPNPSTPLPNTVPQFIAREPYELTVPALYPSSPEVWAPYPLYPAELAPALPPPAFTYPASLHAQCFSPEAKPNTPVFCPLLQQIRFVSGNVFVTYQPTADQQRELPC Predict reactive species |
| Full Name | RNA binding protein with multiple splicing |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC003608 |
| Gene Symbol | RBPMS |
| Gene ID (NCBI) | 11030 |
| RRID | AB_2238431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q93062 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBPMS is a member of the RNA recognition motif family of RNA-binding proteins.It has a single, putative RNA recognition motif in its N-terminus.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RBPMS antibody 15187-1-AP | Download protocol |
| IF protocol for RBPMS antibody 15187-1-AP | Download protocol |
| IHC protocol for RBPMS antibody 15187-1-AP | Download protocol |
| IP protocol for RBPMS antibody 15187-1-AP | Download protocol |
| WB protocol for RBPMS antibody 15187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-RBPMS antibody (Cat# 15187-1-AP) has accumulated 62 citations across 36 journals, including Cell, Nature Communications, Neuron, Circulation Research, Cell Reports, Current Biology, and Advanced Science. Research fields primarily cover neuroscience (retinal ganglion cell degeneration, glaucoma, optic nerve injury), oncology (bladder and ovarian cancer), cardiology (cardiac splicing), and retinal neurodevelopment.
| Species | Application | Title |
|---|---|---|
Cell Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. | ||
Cell Perivascular neurons instruct 3D vascular lattice formation via neurovascular contact | ||
Nat Commun A TLK2-mediated calcium-driven cell death pathway links neuronal degeneration to nuclear envelope disruption | ||
Nat Commun Proteogenomics of different urothelial bladder cancer stages reveals distinct molecular features for papillary cancer and carcinoma in situ
| ||
Nat Commun CRISPR-CasRx-mediated disruption of Aqp1/Adrb2/Rock1/Rock2 genes reduces intraocular pressure and retinal ganglion cell damage in mice | ||
Reviews
What customers say
Customer reviews for this antibody are mixed. One user (5★) reported excellent performance for Western Blot at 1:1000 dilution, noting a very specific band at the expected molecular weight of RBPMS and highly recommending the product. However, another user (3★) found it unsuitable for Immunohistochemistry and Immunofluorescence on paraffin-embedded adult mouse retina, citing poor specificity and significant background staining. Overall, the antibody appears reliable for WB but may require optimization or may not be ideal for IHC/IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christoffer (Verified Customer) (03-03-2025) | Does not stain specific, lots of background.
|
Tom (Verified Customer) (07-26-2022) | This antibody works great for western blot. I had a very specific band at the expected MW of RBPMS. Highly recommended!
|
