Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-15187 targets RBPMS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7256 Product name: Recombinant human RBPMS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC003608 Sequence: MNNGGKAEKENTPSEANLQEEEVRTLFVSGLPLDIKPRELYLLFRPFKGYEGSLIKLTSKQPVGFVSFDSRSEAEAAKNALNGIRFDPEIPQTLRLEFAKANTKMAKNKLVGTPNPSTPLPNTVPQFIAREPYELTVPALYPSSPEVWAPYPLYPAELAPALPPPAFTYPASLHAQCFSPEAKPNTPVFCPLLQQIRFVSGNVFVTYQPTADQQRELPC Predict reactive species |
| Full Name | RNA binding protein with multiple splicing |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC003608 |
| Gene Symbol | RBPMS |
| Gene ID (NCBI) | 11030 |
| RRID | AB_2920227 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q93062 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBPMS is a member of the RNA recognition motif family of RNA-binding proteins.It has a single, putative RNA recognition motif in its N-terminus.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 RBPMS antibody CL647-15187 | Download protocol |
| IF protocol for CL Plus 647 RBPMS antibody CL647-15187 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The product received a 5-star rating for its use in immunofluorescence. The reviewer reported excellent performance when staining mouse retina tissue, indicating strong specificity and reliable signal in this application. Overall, the feedback highlights the antibody's effectiveness for IF work on retinal samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Annu (Verified Customer) (09-18-2025) | It works great on mouse retina.
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