ChromoTek RFP-Booster ATTO594

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

Specificity

mCherry, mPlum, mRFP

Applications

IF, IHC, ICC

Conjugate

ATTO 594

Host

Alpaca

Cat no : rba594

Synonyms

RFP, RFP-Booster, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

DescriptionThe RFP-Booster stabilizes, enhances, and reactivates the signal of RFP-fusion proteins such as mCherry, dsRed, mRFP, etc.. Due to its small size, the RFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, IHC, ICC
HostAlpaca
Specificity/TargetmCherry, mPlum, mRFP, mRFPruby, mKate2
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
ConjugateATTO 594
Physical StateLiquid
Suggested DilutionIF/ICC/IHC: 1:200
Type Nanobody
ClassRecombinant
RRIDAB_2631390
Affinity (KD) Dissociation constant KD of 5nM
Storage Buffer1x PBS, 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for 6 month. Do not freeze. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Front Cell Infect Microbiol

Investigating the effect of bacteriophages on bacterial FtsZ localisation

Authors - Gurneet K. Dhanoa
IF

Int J Mol Sci

Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy

Authors - Kimberly Cramer
IF

Adv Healthc Mater

Cascaded photoinduced drug delivery to cells from multifunctional core-shell mesoporous silica.

Authors - Axel Schlossbauer
IF

Methods Mol Biol

Fluorescent reporters and methods to analyze fluorescent signals.

Authors - Annette Müller-Taubenberger
IF

Nat Struct Mol Biol

A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction.

Authors - Maro Iliopoulou
IF

Methods Enzymol

TRICK: A Single-Molecule Method for Imaging the First Round of Translation in Living Cells and Animals.

Authors - J M Halstead