ChromoTek RFP-Booster Alexa Fluor® 568

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

Ready to use reagent

Specificity

mCherry, mPlum, mRFP

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 568

Cat no : rb2AF568

Synonyms

RFP, RFP-Booster, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

---

Validation Data Gallery

• 

  HeLa cells transiently transfected with PCNA-mRFP and Tom70-eGFP were subjected to one-step immunostaining with RFP-Booster Alexa Fluor 568 (red) and GFP-Booster Alexa Fluor 488 (green). Scale bar, 10 µm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

---

Product Information

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

• Applications: IF, IHC, ICC
• Recommended Dilution: IF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 5 nM
• Specificity/Target: mCherry, mPlum, mRFP, mRFPruby, mKate2
• Conjugate: Alexa Fluor® 568
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light.