ChromoTek RFP-Booster Alexa Fluor® 568

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

Specificity

mCherry, mPlum, mRFP

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 568

Publications

1

Cat no : rb2AF568

Synonyms

RFP, RFP-Booster, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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Validation Data Gallery

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  HeLa cells transiently transfected with PCNA-mRFP and Tom70-eGFP were subjected to one-step immunostaining with RFP-Booster Alexa Fluor 568 (red) and GFP-Booster Alexa Fluor 488 (green). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

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Product Information

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

• Applications: IF, IHC, ICC
• Recommended Dilution: IF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 5 nM
• Specificity/Target: mCherry, mPlum, mRFP, mRFPruby, mKate2
• Conjugate: 0.5 – 1 mg/mL (conjugates)
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light.

Publications

Application	Title
IF	Dev Cell; Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins.; Authors - Ramona Lattao