ChromoTek RFP-Booster Alexa Fluor® 568

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

Specificity

mCherry, mPlum, mRFP

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 568

Host

Alpaca

Publications

4

Cat no : rb2AF568

Synonyms

RFP, RFP-Booster, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag

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Validation Data Gallery

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  HeLa cells transiently transfected with PCNA-mRFP and Tom70-eGFP were subjected to one-step immunostaining with RFP-Booster Alexa Fluor 568 (red) and GFP-Booster Alexa Fluor 488 (green). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

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Product Information

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

• Description: The RFP-Booster stabilizes, enhances, and reactivates the signal of RFP-fusion proteins such as mCherry, dsRed, mRFP, etc.. Due to its small size, the RFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

  • Considerably higher tissue penetration rates

  • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

  • Less than 2 nm epitope-label displacement minimizes linkage error

  • Monovalent VHHs do not cluster their epitopes

  • Validation: structure and function characterized

  • Consistent and reliable performance due to recombinant production

• Applications: IF, IHC, ICC
• Host: Alpaca
• Specificity/Target: mCherry, mPlum, mRFP, mRFPruby, mKate2; For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
• Conjugate: Alexa Fluor® 568
• Physical State: Liquid
• Suggested Dilution: IF/ICC/IHC: 1:500- 1:1,000
• Type: Nanobody
• Class: Recombinant
• DOL: 2 fluorophores per Nanobody
• RRID: AB_2827576
• Affinity (KD): Dissociation constant K_D of 5nM
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA ; Preservative: 0.09% Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
• Note: This product is for research use only, not for diagnostic or therapeutic use

Publications

Application	Title
IF	Cell Rep; Targeting the tamoxifen receptor within sodium channels to block osteoarthritic pain; Authors - Megan M Mccollum
FC	Cell; Sublethal cytochrome c release generates drug-tolerant persister cells; Authors - Halime Kalkavan
IF	Elife; A light-gated transcriptional recorder for detecting cell-cell contacts.; Authors - Kelvin F Cho
IF	Dev Cell; Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins.; Authors - Ramona Lattao