ChromoTek RFP-Booster ATTO647N

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

Specificity

mCherry, mPlum, mRFP

Applications

IF, IHC, ICC

Conjugate

ATTO 647N

Host

Alpaca

Cat no : rba647n

Synonyms

RFP, RFP-Booster, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

anti-RFP VHH/ Nanobody conjugated to fluorophore for IF, of mCherry and other red fluorescent protein-fusion proteins

DescriptionThe RFP-Booster stabilizes, enhances, and reactivates the signal of RFP-fusion proteins such as mCherry, dsRed, mRFP, etc.. Due to its small size, the RFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, IHC, ICC
HostAlpaca
Specificity/TargetmCherry, mPlum, mRFP, mRFPruby, mKate2
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
ConjugateATTO 647N
Physical StateLiquid
Suggested DilutionIF/ICC/IHC: 1:200
Type Nanobody
ClassRecombinant
RRIDAB_2631391
Affinity (KD) Dissociation constant KD of 5nM
Storage Buffer1x PBS, 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for 6 month. Do not freeze. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Geroscience

Pharmacological or genetic depletion of senescent astrocytes prevents whole brain irradiation-induced impairment of neurovascular coupling responses protecting cognitive function in mice.

Authors - Andriy Yabluchanskiy
IF

Int J Mol Sci

Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy

Authors - Kimberly Cramer
IF

Anticancer Res

Administration of Gapmer-type Antisense Oligonucleotides Targeting γ-Glutamylcyclotransferase Suppresses the Growth of A549 Lung Cancer Xenografts.

Authors - Hiromi Ii

Membranes (Basel)

Precise Detection and Visualization of Nanoscale Temporal Confinement in Single-Molecule Tracking Analysis

Authors - Manon Westra
IF

Mol Biol Cell

Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS.

Authors - Falk Schneider
IF

J Biol Chem

LRRC8/VRAC anion channels are required for late stages of spermatid development in mice.

Authors - Jennifer C Lück