|Positive WB detected in||mouse skeletal muscle tissue, mouse heart tissue, human heart tissue, human skeletal muscle tissue, rat skeletal muscle tissue, MCF-7 cells, human heart tissue, human skeletal muscle tissue, MCF-7 cells|
|Positive IHC detected in||human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
11590-1-AP targets RGS5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RGS5 fusion protein Ag2196|
|Full Name||regulator of G-protein signaling 5|
|Calculated molecular weight||181 aa, 21 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC030059|
|Gene ID (NCBI)||8490|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kd band recognized by this antibody is a RGS5-RGS5 dimer. (Pubmed:17762159)
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