RGS5 Polyclonal antibody

RGS5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11590-1-AP

Synonyms

MST092, MST106, MST129, MSTP032, MSTP092, MSTP106, MSTP129, RGS5



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue, human heart tissue, human skeletal muscle tissue, rat skeletal muscle tissue, MCF-7 cells, human heart tissue, human skeletal muscle tissue, MCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

11590-1-AP targets RGS5 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGS5 fusion protein Ag2196
Full Name regulator of G-protein signaling 5
Calculated molecular weight 181 aa, 21 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC030059
Gene symbol RGS5
Gene ID (NCBI) 8490
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kd band recognized by this antibody is a RGS5-RGS5 dimer. (Pubmed:17762159)

Protocols

Product Specific Protocols
WB protocol for RGS5 antibody 11590-1-APDownload protocol
IHC protocol for RGS5 antibody 11590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds.

Authors - Christian F Guerrero-Juarez
mouseWB

Arterioscler Thromb Vasc Biol

HuR (Human Antigen R) Regulates the Contraction of Vascular Smooth Muscle and Maintains Blood Pressure.

Authors - Shanshan Liu
humanIF

Nat Commun

Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.

Authors - Zhaohui Chen
mouseWB,IHC

Cell

Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers.

Authors - Ursula Quitterer