RGS5 Polyclonal antibody

RGS5 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11590-1-AP


MST092, MST106, MST129, MSTP032, MSTP092, MSTP106, MSTP129, RGS5

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue, mouse heart tissue, MCF-7 cells, rat skeletal muscle tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11590-1-AP targets RGS5 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGS5 fusion protein Ag2196
Full Name regulator of G-protein signaling 5
Calculated Molecular Weight 181 aa, 21 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC030059
Gene Symbol RGS5
Gene ID (NCBI) 8490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kd band recognized by this antibody is a RGS5-RGS5 dimer. (Pubmed:17762159)


Product Specific Protocols
WB protocol for RGS5 antibody 11590-1-APDownload protocol
IHC protocol for RGS5 antibody 11590-1-APDownload protocol
IF protocol for RGS5 antibody 11590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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