CoraLite®594-conjugated RPL3 Monoclonal antibody

RPL3 Monoclonal Antibody for WB, IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G1B8

Cat no : CL594-66130

Synonyms

60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B

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Validation Data Gallery

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  WB analysis using CL594-66130

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66130 (RPL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using CL594-66130

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 RPL3 antibody (CL594-66130, Clone: 2G1B8 ) at dilution of 1:200.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66130 is RPL3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPL3 fusion protein Ag20400
• Full Name: ribosomal protein L3
• Calculated Molecular Weight: 46/27 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC012786
• Gene Symbol: RPL3
• Gene ID (NCBI): 6122
• RRID: AB_2919951
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.