CoraLite® Plus 488-conjugated RYR1 Monoclonal antibody

RYR1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B4E12

Cat no : CL488-66539

Synonyms

CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, SKRR

---

Validation Data Gallery

• 

  IF Staining of PC-12 using CL488-66539

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 RYR1 antibody (CL488-66539, Clone: 1B4E12 ) at dilution of 1:100.

• 

  FC experiment of SH-SY5Y using CL488-66539

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human RYR1 (CL488-66539, Clone:1B4E12) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF detected in: PC-12 cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66539 targets RYR1 in IF, FC (Intra) applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RYR1 fusion protein Ag25120
• Full Name: ryanodine receptor 1 (skeletal)
• Calculated Molecular Weight: 565 kDa
• GenBank Accession Number: NM_000540
• Gene Symbol: RYR1
• Gene ID (NCBI): 6261
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.