S100A16 Recombinant monoclonal antibody

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  WB analysis of A431 using 82923-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 82923-1-RR (S100A16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 82923-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A16 Recombinant antibody (82923-1-RR, Clone:3E1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

S100A16 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 82923-1-RR

Clone No.3E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

3E1, AAG13, Aging-associated gene 13 protein, Protein S100 A16, Protein S100 F

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Tested Applications

• Positive WB detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82923-1-RR targets S100A16 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2023

  Product name: Recombinant human S100A16 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-103 aa of BC019099

  Sequence: MSDCYTELEKAVIVLVENFYKYVSKYSLVKNKISKSSFREMLQKELNHMLSDTGNRKAADKLIQNLDANHDGRISFDEYWTLIGGITGPIAKLIHEQEQQSSS

• Full Name: S100 calcium binding protein A16
• Calculated Molecular Weight: 103 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC019099
• Gene Symbol: S100A16
• Gene ID (NCBI): 140576
• RRID: AB_3670658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96FQ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A16 is a member of the S100 protein family. S100A16 is expressed in a variety of human tissues. S100A16 expression is especially high in tissues rich in epithelial cells. Functionally, S100A16 has been linked to several aspects of tumorigenesis, for example, cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition (EMT).Accordingly, S100A16 has been suggested to have both tumour-promoting and suppressive roles in human cancers. S100A16-mediated cellular functions are suggested to be mediated by the regulation of various signaling pathways/proteins including EMT-related proteins E-cadherin and Vimentin, PI3K-AKT, p53, MMP1-1, MMP-2, MMP-9, JNK/p38, etc. (PMID: 37509106).