Sam50 Monoclonal antibody
Sam50 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Pig, Rat, Mouse, rabbit
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2H6H9
Cat no : 67425-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A375 cells, rabbit heart tissue, HSC-T6 cells, NIH/3T3 cells, pig heart tssue, rat heart tissue, mouse heart tissue |
Positive IHC detected in | human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67425-1-Ig targets Sam50 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Rat, Mouse, rabbit samples.
Tested Reactivity | Human, Pig, Rat, Mouse, rabbit |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Sam50 fusion protein Ag15155 |
Full Name | sorting and assembly machinery component 50 homolog (S. cerevisiae) |
Calculated Molecular Weight | 469 aa, 52 kDa |
Observed Molecular Weight | 52 kDa |
GenBank Accession Number | BC007830 |
Gene Symbol | SAMM50 |
Gene ID (NCBI) | 25813 |
RRID | AB_2882664 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).
Protocols
Product Specific Protocols | |
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WB protocol for Sam50 antibody 67425-1-Ig | Download protocol |
IHC protocol for Sam50 antibody 67425-1-Ig | Download protocol |
IF protocol for Sam50 antibody 67425-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |